At the moment I can often be found staring at movies of dividing microorganisms.
We are investigating how growth and gene expression regulation are intertwined at a single cell level, to better understand how physiological processes impact phenotypic realizations. In addition, we would like to know to what extent fundamental growth laws derived from population-level measurements apply to individual cells. To this end, we are employing timelapse microscopy and are developing a generic pipeline for monitoring, segmenting and tracking of individual cells in dense bacterial cultures.
Non-genetic origins of phenotypic variability
Coordination of growth and metabolism
Dynamic (fast) and adaptive (slow) aspects of metabolic regulation
Single-cell measurement techniques
Developing and improving experimental methodologies
I obtained my B.Sc. (2006) and M.Sc. (2009) degrees in molecular cell biology from the University of Cape Town (South Africa). I started my research career by investigating the potential to exploit gene expression profiles of peripheral tissues as non-invasive diagnostic indicators of psychiatric states, which culminated in my M.Sc. dissertation. In 2009 I joined the group of Prof. Bas Teusink as a PhD student, venturing into the world of microbial physiology and metabolism. In this position, I researched the dynamic regulation of glycolysis in baker’s yeast, focusing on the role that trehalose metabolism plays during transitions from low to high glucose environments.
Currently, as a researcher in the group of Prof. Frank Bruggeman, I am trying to understand how the processes of growth and division impact the stability and variability of phenotypic traits in microbial populations.
Vrije Universiteit Amsterdam
De Boelelaan 1108, 1081 HZ, Amsterdam
Faculty of Earth and Life Science
Room O|2 01W55
tel: +31 (0)20 598 6993
mail: please click my photo
Iraes Rabbers, Johan H. van Heerden, Niclas Nordholt, Herwig Bachmann, Bas Teusink, Frank J. Bruggeman (2015). Metabolism at evolutionary optimal States. Metabolites (2):3
JH van Heerden, FJ Bruggeman, B Teusink (2015). Multi‐tasking of biosynthetic and energetic functions of glycolysis explained by supply and demand logic. BioEssays 37 (1), 34-45
Johan H van Heerden, Meike T Wortel, Frank J Bruggeman, Joseph J Heijnen, Yves JM Bollen, Robert Planqué, Josephus Hulshof, Tom G O’Toole, S Aljoscha Wahl, Bas Teusink, (2014). Lost in Transition: Startup of Glycolysis Yields Subpopulations of Nongrowing Cells. Science, 1245114
Johan H van Heerden, Meike T Wortel, Frank J Bruggeman, Joseph J Heijnen, Yves JM Bollen, Robert Planqué, Josephus Hulshof, Tom G O’Toole, S Aljoscha Wahl, Bas Teusink, (2014). Fatal attraction in glycolysis: how Saccharomyces cerevisiae manages sudden transitions to high glucose. Microbial Cell 1 (3), 103-106
JH van Heerden, V Russell, A Korff, DJ Stein, N Illing, (2010). Evaluating the behavioural consequences of early maternal separation in adult C57BL/6 mice; the importance of time. Behavioural brain research 207 (2), 332-342
DS Williamson, KC Dent, BW Weber, A Varsani, J Frederick, RN Thuku, RA Cameron, JH van Heerden, DA Cowan, BT Sewell, (2010). Structural and biochemical characterization of a nitrilase from the thermophilic bacterium,Geobacillus pallidus RAPc8. Appl. Microbiol. Biotechnol. 88:143-153 [DOI] [PubMed]
J van Heerden, A Conesa, D Stein, D Montaner, V Russell, N Illing, (2009). Parallel changes in gene expression in peripheral blood mononuclear cells and the brain after maternal separation in the mouse. BMC research notes 2 (1), 195
J van Heerden, SA Walford, A Shen, N Illing, (2007). A framework for the informed normalization of printed microarrays. South African Journal of Science 103 (9-10), 381-390
N. Illing, S. Walford, A. Shen, J. van Heerden, N. Immelman, N. Mulder, DK. Denby, (2007). Molecular tools to study desiccation tolerance in the resurrection plant Xerophyta humilis. South African J. Bot. 73:487-487
Supervision of students
|Ioanna Chatzigiannidou||Frank Bruggeman||Modulating stress susceptibility in E. coli through environmental conditioning||MSc||2015|
|Coco van Boxtel||Frank Bruggeman||Implementing and evaluating Spinach2 for in vivo flourescence based monitoring of intracellular ADP levels in E. coli||MSc||2015|
|Joyce Lam||Bas Teusink/Joachim Goedhart||In vitro characterizing of the pH-dependence of FRET-sensors for glycoltyic intermediates||MSc||2014|
|Phillipp Schmidt||Frank Bruggeman/Anne Schwabe||Quantifying the reciprocal regulation of the PFK/FBP enzyme pair in yeast, using RNA FISH||MSc||2014|
|Valentijn Jansen||Bas Teusink||Evaluting the pH dependence of glycolytic enzyme activities||BSc||2013|
|Wen Korrel||Bas Teusink||Implementing FRET-sensors for the in vivo quantification of glycolytic metabolites in S. cerevisiae||BSc||2013|
|Sandra Windhorst||Bas Teusink||Intracellular pH dynamics of tps1- mutants||BSc||2011|
|Bahar Ramezanpour||Bas Teusink||Glucose tolerance of tps1- mutants||BSc||2010|