New review on spatiotemporal coupling of gene expression published

We combined our expertise in Gene expression regulation, Single-molecule RNA imaging, and Prokaryotic quantitative cell physiology to write a truly multidisciplinary review. It’s the product of a team effort by Alan Gerber (Amsterdam UMC), Sander van Otterdijk (Sysbio),  Frank Bruggeman (Sysbio) and Evelina Tutucci (Sysbio).

We discussed state-of-the-art imaging approaches for the measurement and quantitative understanding of gene expression, starting from the early visualizations of single genes by electron microscopy to current fluorescence-based approaches in single cells, including live-cell RNA-imaging approaches to FISH-based spatial transcriptomics across model organisms. We also highlighted how these methods have shaped our understanding of the spatiotemporal coupling between transcription and translation in prokaryotes and the future challenges in the field.

We had a lot of fun preparing some nice figures too, which illustrate our growing interest in the spatial control of gene expression across kingdoms of life.

The review “Understanding spatiotemporal coupling of gene expression using single molecule RNA imaging technologies” is now published in Transcription (

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